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Mitragynine: The most abundant alkaloid, responsible for energy-boosting effects 7-hydroxymitragynine: More potent than mitragynine, contributes to pain relief Speciogynine: May have muscle relaxant properties Paynantheine: Believed to work synergistically with other alkaloi

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The most reliable method for determining alkaloid content is high-performance liquid chromatography (HPLC). This technique separates and identifies individual compounds, allowing for precise quantification of specific alkaloids. Mass spectrometry can also be used in conjunction with HPLC for enhanced accurac

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